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![]() Do not heat, dilute or add reducing agent before loading.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25☌), 2% SDS, 0.2mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol). It is also advisory to double check that any experimental manipulations do not affect its levels. The ladder is supplied in gel loading buffer and is ready to use. However, some caution is advised when selecting this protein for Western blot detection as many other proteins run at its 17kDa size during SDS-PAGE (make sure your band of interest won’t be obscured). Our Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Enzo's Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Learn more about the relative transfer efficiencies of tank, semi-dry, and rapid blotting systems for western blotting with large proteins.
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